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Labs owning dPCR and susceptible to participate to JRC stability studies

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Dear Colleagues,

As you know, the validated PCR methods for the detection of ruminant, pig and poultry DNA in feed need the determination of a specific cut-off for each PCR platform used for that purpose. The determination of the cut-off is based on the use of calibrants at well-defined copy numbers provided by JRC. To insure the quality of the calibrants, JRC organizes characterisation studies during which the copy numbers concentrations of the calibrants are determined by different labs using different digital (chamber or droplet) PCR platforms. Also regular stability studies are organised for which digital PCR measurements are needed.

JRC would like to extend its panel of labs participating to these studies and asked to EURL-AP to diffuse this call.

Labs using digital PCR and interested to participate to the studies organised by JRC can visit the webpage Participation in CRM projects – Certified Reference Materials catalogue of the JRC (europa.eu) for more information.

Thank you for your interest and support,

Best regards,

Calibration kit (calibrants) for poultry detection by PCR available

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The EURL-AP is pleased to announce that the ERM-AD484k POULTRY pDNA CALIBRANT is now available and can be ordered. To access to the right page of the JRC website click here Search (484k) – ERM-AD484k POULTRY pDNA CALIBRANT ” – Certified Reference Materials catalogue of the JRC (europa.eu).

You can also find a link on the EURL-AP website. Go to https://www.eurl.craw.eu/legal-sources-and-sops/method-of-reference-and-sops/. In the section “Calibration kits for ruminant, pig and poultry detection by PCR”, click on the dedicated link.

Pig cut-off Excel file

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With the new batch of pig calibrants now commercially available, you will have to adapt the exact copy numbers to be in accordance with the certificate provided with these new calibrants.

In this “Pig cut-off at 5 copies” Excel file, the cells where you have to introduce the “exact copy numbers” are not locked and this modification is possible.

Do not forget that the figures mentioned in the certificate are copies/µl! In the cut-off Excel file, you have to introduce the number of copies/5 µl!

Calibration kits for ruminant and pig detection by PCR commercially available now

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The calibration kits ERM-AD482 RUMINANT pDNA CALIBRANT and ERM-AD483 PORCINE pDNA CALIBRANT can be ordered from JRC-IRMM. They are now Certified European Reference Materials.

 For ordering and information, go to the following webpages : 

https://crm.irmm.jrc.ec.europa.eu/p/q/erm-ad482+/ERM-AD482-RUMINANT-pDNA-CALIBRANT/ERM-AD482

https://crm.irmm.jrc.ec.europa.eu/p/q/erm-AD483/ERM-AD483-PORCINE-pDNA-CALIBRANT/ERM-AD483

 

EURL-AP PT PCR 2015 : report form (version Excel 2010) available

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Dear NRL Colleagues,

The Excel file containing the instructions, report form and summary is available for download on the intranet document library. Two versions of the document are available: Excel 97-2003 and Excel 2010. Be free to use the most convenient file for you. We wish you a succesful participation.

EURL-AP PCR proficiency test 2014

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Official announcement for the next EURL-AP Proficiency Test 2014 (PCR)

Dear NRL Colleagues,

Please find in attachement of this post the official announcement for the EURL-AP Proficiency Test 2014 (PCR) that will be organised in April.

In the announcement you will find the time schedule.

Best Regards,